Abstract: Objective　To investigate the effects of povidone-iodine immersion treatment on the embryonic development of red crucian carp (Carassius auratus), and explore its blocking effect on Cyprinid herpesvirus 2 (CyHV-2), providing a technical solution for viral eradication in experimental animal populations of red crucian carp.Methods　Clinically abnormal individuals were selected from a closed red crucian carp population. Viruses were isolated using cell culture method, and viral species were identified through PCR amplification and DNA sequencing. Gradient concentrations of povidone-iodine solution (10.0, 20.0, 30.0, 40.0 mg/mL) were applied for 5-minute immersion treatment on fertilized eggs. Safe and effective treatment protocols were screened by statistically analyzing embryonic hatching rates and malformation rates. Treated individuals at different developmental stages were sampled periodically, and CyHV-2 infections were tracked using PCR technology. Results　Viruses were successfully isolated from two diseased individuals in a closed population aquarium and confirmed as CyHV-2 through molecular biological identification. Experiments showed that povidone-iodine treatments at concentrations of 10.0, 20.0, 30.0, and 40.0 mg/mL for 5 minutes did not significantly affect embryonic development. No CyHV-2 virus was detected in any period of the group treated with 40.0 mg/ mL povidone-iodine for 5 minutes.Conclusion　Povidone-iodine treatment of red crucian carp fertilized eggs can block the transmission of CyHV-2 and could be developed into a standard method for viral eradication in experimental fish populations.

Key words: red crucian carp, cyprinid herpesvirus type 2, povidone-iodine, virus blocking

摘要： 目的　研究聚维酮碘浸泡处理对红鲫胚胎发育的影响,探究其对鲤疱疹病毒 Ⅱ 型(CyHV-2)的阻断作用,为 红鲫实验动物种群的病毒净化提供技术方案。方法　采用细胞培养法从红鲫封闭种群的临床症状异常个体中分 离病毒,结合 PCR 扩增与 DNA 测序技术鉴定病毒种类;设置 10.0、20.0、30.0、40.0 mg/mL 梯度浓度的聚维酮碘 溶液,对红鲫受精卵进行 5 min浸泡处理,通过统计各组胚胎孵化率及畸形率,筛选安全有效的处理方案;定期采集 不同发育阶段的处理个体样本,利用 PCR 技术追踪检测 CyHV-2 的感染。结果　从红鲫封闭群某水族箱的 2 尾患 病个体中成功分离到病毒,经分子生物学鉴定确认为 CyHV-2;实验证明10.0、20.0、30.0、40.0 mg/mL等不同浓度 聚维酮碘处理受精卵5 min均未显著影响胚胎发育,在40.0 mg/mL聚维酮碘5 min处理组不同时期均没有检测到 CyHV-2病毒。结论　红鲫受精卵的聚维酮碘处理可阻断CyHV-2的传播,或可发展为标准方法用于鱼类实验动物 的病毒净化。

关键词: 红鲫, 鲤疱疹病毒2型, 聚维酮碘, 病毒阻断